Chapter 8 in “Cryobiology for South American Neotropical fish species”, 170-182. 2024.
New Publication: Embryos and Ovarian Tissues of South American Neotropical Fish: Current State of Development.
Cryopreservation is a biotechnology used to conserve living cells, gametes, and embryos during a short or prolonged storage time, using liquid nitrogen as a refrigerant medium. Its mechanism is based on achieving cell dehydration in a controlled manner, allowing intracellular and extracellular fluid exchange, thus mitigating the formation of ice crystals in the cytosol (Chao and Liao 2001).