Abiotic and biotic stress in plants Laboratory
Heads
- Oscar A. Ruiz, PhD - Superior Researcher CONICET. Associate Professor UNSAM ruiz@intech.gov.ar
Members
- Andrés Gárriz, PhD - Independent Researcher CONICET. Adjunct Porfessor UNSAM, garriz@intech.gov.ar
- Franco R. Rossi, PhD - Adjunct Researcher CONICET . Teaching Assistant UNSAM francorossi@intech.gov.ar
- Fernando M. Romero, PhD - Assistant Research CONICET mromero@intech.gov.ar
- Leandro Solmi, Grad - Doctoral fellow CONICET leosolmi@intech.gov.ar
- Maximiliano Gortari, Eng - Doctora fellow CONICET mgortari@intech.gov.ar
- Agr. Eng Doctoral Fellow ANPCyT mestieben@intech.gov.ar
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Claudia Mariam Torres Fernández, MSc. - Becaria Doctoral fellow - CONICET - ctorres@intech.gov.ar
Personal de Apoyo
Introduction
What are the causes of stress in plants? What are abiotic or biotic stresses? As in other organisms, plant stress can be originated from changes in the environmental conditions (abiotic stress), or as a product of the interactions established by plants with organisms such as fungi, bacteria, and virus (biotic stress). In this trend, the presence of high levels of salts (salt stress), the absence (water stress) or the excess (flood stress) of water, and changes in the temperatures conduce to abiotic stress, as well as the excess of agrochemicals such as fertilizers and pesticides. In turn, biotic stress is caused by pathogenic microorganisms that invade plant tissues originating wilting of plants, leaf spots, root rot or damages in seeds. However, there is a group of invading microorganisms that cause beneficial effects on plants, increasing their tolerance to stress and/or promoting their growth and development.
Our projects try to understand the molecular bases that explain the responses of plants to stress, and use this knowledge to optimize management strategies of crops with agronomic interest, enhancing plant production without causing damage to the environment:
Publications
Campestre MP, Antonelli CJ, Bailleres MA, Gortari M, Maguire VG, Ezquiaga JP, Taboada MA, Ruiz OA. An efficiently biological nitrogen fixation of non-native Lotus tenuis justifies its key role in the flooding Pampas (Argentina). Farming System 3, 100122. 2025. https://doi.org/10.1016/j.farsys.2024.100122
Arese RP, Gortari M, Ezquiaga JP, Illanes FA, Ruiz OA. Efectos de un aditivo dietario tanífero en el control bioactivo de la haemonchosis ovina. Brazilian Journal of Animal and Environmental Research 7, 2. 2024. https://doi.org/10.34188/bjaerv7n2-122
Campestre MP, Antonelli CJ, Castagno NL, Maguire VG, Ruiz OA. Interspecific hybridization and inoculation with Pantoea eucalypti improve forage performance of Lotus crop species under alkaline stress. Plant Biology 26, 143-345. 2024. https://doi.org/10.1111/plb.13614
Corvi MM, Rossi F, Ganuza A, Alonso AM, Alberca LN, Dietrich RC, Gavernet L, Talevi A. Triclabendazole and clofazimine reduce replication and spermine uptake in vitro in Toxoplasma gondii. Parasitology Research 123, 69. 2024. https://doi.org/10.1007/s00436-023-08062-4
Fernández PV, Vago ME, Ezquiaga JP, Maiala S, Rodriguez A, Acosta JM, Gortari M, Ruiz OA, Ciancia M. Plant Stress 100519. 2024. https://doi.org/10.1016/j.stress.2024.100519
Recalde L, Cabrera AV, Gómez Manzur NM, Rossi FM, Groppa MD, Benavidez MP. Seed priming with spermine improves early wheat growth under Nitrogen deficiency. Journal of Plant Growth Regulation 43, 3761–3775. 2024. https://doi.org/10.1007/s00344-024-11360-5
Ruiz OA, Gortari M, Maguire VG, Arese RP, Campestre MP, Antonelli CJ, Calzadilla PI, Menéndez AB, Escaray FJ, Carrasco Sorli PM, Bailleres MA, Ezquiaga JP, Paolocci F, Gárriz A, Nieva AS. Lotus spp.: a Mediterranean genus with high environment and economic impact in the Salado River Basin (Argentina). Discover Life 54, 3. 2024. https://doi.org/10.1007/s11084-024-09646-5
Van de Wouw AP, Scanlan JL, Al-Mamun HA, Balesdent MH, Bousset L, Burketová L, del Rio Mendoza L, Dilantha Fernando WG, Franke C, Howlett BJ, Huang YJ, Jones EE, Koopman B, Lob S, Mirabadi AZ, Nugent B, Peng G, Rossi FR, Schreuder H, Tabone AR, Van Coller GJ, Batley J, Idnurm A. A new set of international Leptosphaeria maculans isolates as a resource for elucidation of the basis and evolution of blackleg disease on Brassica napus. Plant Pathology 73, 170-185. 2024. https://doi.org/10.1111/ppa.13801
Avico EH, Acevedo RM, Duarte MJ, Rodrigues Salvador A, Nunes-Nesi A, Ruiz OA, Sansberro PA. Integrating Transcriptional, Metabolic, and Physiological Responses to Drought Stress in Ilex paraguariensis Roots. Plants 12, 2404. 2023. https://doi.org/10.3390/plants12132404
Escaray FJ, Valeri MC, Damiani F, Ruiz OA, Carrasco P, Paolocci F. Multiple bHLH/MYB-based protein complexes regulate proanthocyanidin biosynthesis in the herbage of Lotus spp. Planta 259. 10. 2023. https://doi.org/10.1007/
Monteoliva MI, Ruiz OA, Li F. Editorial: Legumes and their microbiome in climate change mitigation. Frontiers in Plant Science 14. 2023. https://doi.org/10.3389/fpls.2023.1220535
Soares R, Fareleira P, Colavolpe B, Ruiz OA, Videira e Castro I. Root nodule bacteria isolated from Lotus uliginosus for future use in phytostabilization of arsenic contaminated soils. Grass Research 3, 8. 2023. https://doi.org/10.48130/GR-2023-0008
Solmi L, Rossi FR, Romero MF, Bach-Pages M, Preston GM, Ruiz OA, Gárriz A. Polyamine-mediated mechanisms contribute to oxidative stress tolerance in Pseudomonas syringae. Scientific Reports 13, 4279. 2023. https://doi.org/10.1038/s41598-023-31239-x
Campestre MP, Antonelli CJ, Bailleres MA, Gortari M, Maguire VE, Taboada MA, Ruiz OA. Lotus tenuis biological nitrogen fixation and performance contribute to defining its strategic role in the Salado River Basin lowlands (Argentina). Agriculture, Ecosystems & Environment 340, 108159. 2022. https://doi.org/10.21203/rs.3.rs-2037673/v1
Dinolfo MI, Martínez M, Castañares E, Vanzetti LS, Rossi F, Stenglein SA, Arata AF. Interaction of methyl-jasmonate and Fusarium poae in bread wheat. Fungal Biology 126, 786-798. 2022. https://doi.org/10.1016/j.funbio.2022.10.002
Duhalde MA, Bezus R, Rodríguez AA, Maiale SJ, Romero FM. Optimization of tissue culture conditions and in vitro and in planta transformation of the local variety of rice Don Justo FCAyF. Revsita Facultad Agronomía, Biotecnologías Aplicadas a Cultivos de Interés Socioeconómico 121, 1-13. 2022. https://doi.org/10.24215/
Liebrenz K, Gómez C, Brambilla S, Frare R, Stritzler M, Maguire V, Ruiz O, Soldini D, Pascuan D, Soto G, Ayub N. Whole-Genome resequencing of spontaneous oxidative stress-resistant mutants reveals an antioxidant system of Bradyrhizobium japonicum involved in soybean colonization. Microbial Ecology 84, 113-1140.2022. https://doi.org/10.1007/s00248-021-01925-2
Maguire VG, Rodríguez AA, Ezquiaga JP, Salas N, Gortari M, Ayub N, Bouilly PJ. Romero MF, Gárriz A, Ruiz OA. Analysis of the contribution of Lotus corniculatus to soil carbon content in a rice-pasture rotation system. Agriculture, Ecosystems and Environment 340, 108159. 2022. https://doi.org/10.1016/j.agee.2022.108159
Salas N, Cóceres MV, dos Santos Melo T, Pereira-Neves A, Maguire VG, Rodriguez T, Sabatke B, Ramirez MI, Sha J, Wohlschlegel JA, de Miguel N. VPS32, a member of the ESCRT complex, modulates adherence to host cells in the parasite Trichomonas vaginalis by affecting biogenesis and cargo sorting of released extracellular vesicles. Cellular and Molecular Life Sciences 79, 11. 2022. https://doi.org/10.1007/
Solmi L, Rosli HG, Pombo MA, Stadler S, Rossi FR, Romero FM, Ruiz OA, Gárriz A. Inferring the role of the metabolism of polyamines in the phytopathogenic bacteria Pseudomonas Syringae: a meta-analysis approach Frontiers in Microbiology 13, 893626. 2022. https://doi.org/10.3389/fmicb.2022.893626
Zhang X, Pramod K, Puchalski P, Leehan JD, Rossi FR, Okumoto S, PIlot G, Danna CH. MAMP-elicited changes in amino acid transport activity contribute to restricting bacterial growth. Plant Physiology 189, 2315-2331. 2022. https://doi.org/10.
Antonelli CJ, Calzadilla PI, Campestre MP, Escaray FJ, Ruiz OA. Contrasting response of two Lotus corniculatus L. accessions to combined waterlogging–saline stress. Plant Biology 23, 363–374. 2021. https://doi.org/10.1111/plb.13216
Cumpa-Velásquez LM, Moriconi JI, Dip DP, Castagno LN, Puig ML, Maiale SJ, Santa María GE, Sannazzaro AI, Estrella MJ. Prospecting phosphate solubilizing bacteria in alkaline-sodic environments reveals intra-specific variability in Pantoea eucalypti affecting nutrient acquisition and rhizobial nodulation in Lotus tenuis. Applied Soil Ecology 168, 104125. 2021. https://doi.org/10.1016/j.
Mantz GM, Rossi FR, Viretto PE, Noelting MC, Maiale SJ.Stem canker caused by Phomopsis spp. Induces changes in polyamine levels and chlorophyll fluorescence parameters in pecan leaves. Plant Physiology and Biochemistry 166, 761-769. 2021. https://doi.org/10.1016/j.
Menéndez AB, Ruiz OA. Stress-regulated elements in Lotus spp., as a possible starting point to understand signalling networks and stress adaptation in legumes. PeerJ – Life and Environment. 9, e12110. 2021. https://doi.10.7717/peerj.12110
Nieva AS, Romero FM, Erban A, Carrasco P, Ruiz OA, Kopka J. Metabolic profiling and metabolite correlation network analysis reveal that Fusarium solani Induces differential metabolic responses in Lotus japonicus and Lotus t
Puig ML, Rodríguez AA, Vidal AA, Bezus R, Maiale SJ. Patterns of physiological parameters and nitrogen partitioning in flag leaf explain differential grain protein content in rice. Plant Physiology and Biochemistry 168, 457-464. 2021. https://doi.org/10.1016/
Rossi FR, Gárriz A, Marina M, Pieckenstain FL. Modulation of polyamine metabolism in Arabidopsis thaliana by salicylic acid. Physiologia Plantarum 173, 843-855. 2021. https://doi.org/10.1111/
Antonelli CJ, Calzadilla PI, Campestre MP, Escaray FJ, Ruiz OA. Contrasting response of two Lotus corniculatus L. accessions to combined waterlogging–saline stress. Plant Biology. 13216. 2020. https://doi:10.1111/plb.13216.
Bailleres MA, Campestre MP, Antonelli CJ, Melani GH, Menéndez AB, Ruiz OA. Promotion of Lotus tenuis and calf early weaning as a good management practice for breeding herds in marginal soils of the Flooding Pampa (Argentina). Revista de Investigaciones Agropecuarias 46,267-274. 2020.
Bejerman N, Acevedo RM, de Breuil S, Ruiz OA, Sansberro P, Dietzgen RG, Nome C, Debat H. Molecular characterization of a novel cytorhabdovirus with a unique genomic organization infecting yerba mate (Ilex paraguariensis) in Argentina. Archives of Virology 165, 1475-1479.2020. https://doi.org/10.1007/s00705-020-04609-3
Colavolpe MB, Silva MDC, Maguire VG, Costa A, Videira e Castro I, Ruiz, OA. Antagonistic compounds from controversial bacteria with suppressing effects on the diseases caused by Phytophthora cinnamomi. Arch Phytopatol Pfl En prensa. https://doi.org/10.1080/03235408.2020.1719007
Gázquez A, Abdelgawad H, Baggerman G, Van Raemdonck G, Asard H, Maiale SJ, Rodríguez AA, Beemster GTS. Redox homeostasis in the growth zone of the rice leaf plays a key role in cold tolerance. J Exp Bot. 71: 1053-1066. https://doi.org/10.1093/jxb/erz455.
Maguire V, Bordenave CD, Nieva AS, Llames ME, Colavolpe MB, Gárriz A, Ruiz OA. Soil bacterial and fungal community structure of a rice monoculture and rice-pasture rotation systems. Appl Soil Ecol 151, 103535. 2020. https://doi.org/10.1016/j.apsoil.2020.103535
Pires N, Maiale S, Venturino A, Lascano C. Differential effects of azinphos-methyl and chlorpyrifos on polyamine oxidative metabolism during the embryonic development of Rhinella arenarum and its relation to oxidative stress. Pestic Biochem Phys. 163: 14-22. 2020. https://doi.org/10.1016/j.pestbp.2019.10.007
Acevedo RM, Avico EH, González S, Salvador AR, Rivarola M, Paniego N, Nunes-Nesi A, Ruiz OA, Sansberro PA. Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves. Planta. 250: 445-462. 2019. https://doi.org/10.1007/s00425-019-03178-3
Antonelli CJ, Calzadilla PI, Vilas JM, Campestre MP, Escaray FJ, Ruiz OA. Physiological and anatomical traits associated with tolerance to long-term partial submergence stress in the Lotus genus: responses of forage species, a model and an interspecific hybrid. J Agron Crop Sci. 205: 65-76. 2019. https://doi.org/10.1111/jac.12303
Bordenave CD, Granados Mendoza C, Jiménez Bremont JF, Gárriz A, Rodríguez AA. Defining novel plant polyamine oxidase subfamilies through molecular modeling and sequence analysis. BMC Evol Biol. 19: 28. 2019. https://doi.org/10.1186/s12862-019-1361-z
Bordenave CD, Rocco R, Maiale SJ, Campestre MP, Ruiz OA, Rodriguez AA, Menendez AB. Chlorophyll a fluorescence analysis reveals divergent photosystem II responses to saline, alkaline and saline–alkaline stresses in the two Lotus japonicus model ecotypes MG20 and Gifu-129. Acta Physiol Plant. 41: 167. 2019. https://doi.org/10.1007/s11738-019-2956-0
Calzadilla PI, Vilas JM, Escaray FJ, Unrein F, Carrasco P, Ruiz OA. The increase of photosynthetic carbon assimilation as a mechanism of adaptation to low temperature in Lotus japonicus. Sci Rep. 9: 863. 2019. https://doi.org/10.1038/s41598-018-37165-7
Escaray FJ, Antonelli CJ, Carrasco P, Ruiz OA. Interspecific hybridization improves the performance of Lotus spp. under saline stress. Plant Sci. 283: 202-210. 2019. https://doi.org/10.1016/j.plantsci.2019.02.016
Escaray FJ, Antonelli CJ, Copello GJ, Puig S, Peñarrubia L, Ruiz OA, Perea-García A. Characterization of the copper transporters from Lotus spp. and their Involvement under flooding conditions. Int J Mol Sci. 20: 3136. 2019. https://doi.org/10.3390/ijms20133136
Espasandin FD, Brugnoli EA, Ayala PG, Ruiz OA, Sansberri P. Long-term preservation of Lotus tenuis adventitious buds. Plant Cell Tiss Organ Cult. 136: 373-382. 2019. https://doi.org/10.1007/s11240-018-1522-6
Gázquez A, AbdElgawad H, Baggerman G, Van Raemdonck G, Asard H, Maiale SJ, Rodríguez AA, Beemster GTS. Redox homeostasis in the rice leaf growth zone plays a key role in cold tolerance. J Exp Bot erz455. 2019. https://doi.org/10.1093/jxb/erz455
Menéndez AB, Calzadilla PI, Sansberro PA, Espasandin FD, Gazquez A, Bordenave CD, Maiale SJ, Rodríguez AA, Maguire VG, Campestre MP, Garriz A, Rossi FR, Romero FM, Solmi L, Salloum MS, Monteoliva MI, Debat JH, Ruiz OA. Polyamines and Legumes: Joint Stories of Stress, Nitrogen Fixation and Environment. Front Plant Sci 10: 1415. 2019. https://doi.org/10.3389/fpls.2019.01415
Nieva AS, Vilas JM, Gárriz A, Maiale SJ, Menéndez AB, Erban A, Kopka J, Ruiz OA. The fungal endophyte Fusarium solani provokes differential effects on the fitness of two Lotus species. Plant Physiol Biochem. 144: 100-109. 2019. https://doi.org/10.1016/j.plaphy.2019.09.022
Pires N, Maiale S, Venturino A, Lascano C. Differential effects of azinphos-methyl and chlorpyrifos on polyamine oxidative metabolism during the embryonic development of Rhinella arenarum and its relation to oxidative stress. Pestic Biochem Phys. En Prensa. 2019. https://doi.org/10.1016/j.pestbp.2019.10.007
Romero FM, Gatica-Arias A. CRISPR/Cas9: Development and Application in Rice Breeding. Rice Sci. 26: 265-281. 2019. https://doi.org/10.1016/j.rsci.2019.08.001
Romero FM, Rossi FR, Gárriz A, Carrasco P, Ruíz OA. A bacterial endophyte from apoplast fluids protects canola plants from different phytopathogens via antibiosis and induction of host resistance. Phytopathology 109: 375-383. 2019. https://doi.org/10.1094/PHYTO-07-18-0262-R