Abiotic and biotic stress in plants Laboratory

Heads

Oscar A. Ruiz, PhD - Superior Researcher CONICET. Associate Professor UNSAM ruiz@intech.gov.ar

Dr. Andrés Rodríguez - Adjunct Researcher CONICET. Teaching assistant UNSAM andresrodriguez@intech.gov.ar

Members

Andrés Gárriz, PhD - Adjunct Researcher CONICET. Adjunct Porfessor UNSAM, garriz@intech.gov.ar

Franco R. Rossi, PhD - Adjunct Researcher CONICET . Teaching Assistant UNSAM francorossi@intech.gov.ar

Fernando M. Romero, PhD - Assistant Research CONICET mromero@intech.gov.ar

Santiago Maiale MAIALE, PhD - Adjunct Researcher CONICET. Teaching Assistant UNSAM smaiale@intech.gov.ar.

Leandro Solmi, Grad - Doctoral fellow CONICET leosolmi@intech.gov.ar

Andy Salazar Tamacho, Grad - Doctoral fellow CONICET salazarandy310@gmail.com

Maximiliano Gortari, Eng - Doctora fellow CONICET mgortari@intech.gov.ar

Agr. Eng Doctoral Fellow ANPCyT mestieben@intech.gov.ar

Claudia Mariam Torres Fernández, MSc. - Becaria Doctoral fellow - CONICET - ctorres@intech.gov.ar

Personal de Apoyo

Beatriz Liliana Wyss, Technical CONICET. beatriz.wyss@intech.gov.ar

Introduction

What are the causes of stress in plants? What are abiotic or biotic stresses? As in other organisms, plant stress can be originated from changes in the environmental conditions (abiotic stress), or as a product of the interactions established by plants with organisms such as fungi, bacteria, and virus (biotic stress). In this trend, the presence of high levels of salts (salt stress), the absence (water stress) or the excess (flood stress) of water, and changes in the temperatures conduce to abiotic stress, as well as the excess of agrochemicals such as fertilizers and pesticides. In turn, biotic stress is caused by pathogenic microorganisms that invade plant tissues originating wilting of plants, leaf spots, root rot or damages in seeds. However, there is a group of invading microorganisms that cause beneficial effects on plants, increasing their tolerance to stress and/or promoting their growth and development.

Our projects try to understand the molecular bases that explain the responses of plants to stress, and use this knowledge to optimize management strategies of crops with agronomic interest, enhancing plant production without causing damage to the environment:

Research Lines

Effect of suboptimal temperatures on the production of rice (Oryza sativa). (Dr. Rodríguez).

Molecular interactions between plants and microorganisms. Role of the polyamine metabolism (Dr. Gárriz/Dr. Rossi).

Legumes and livestock production in the Salado River Basin. Productive aspects, soil improvement strategies and contribution to the environment (Dr. Ruiz)

Nutrition and improvement of rice (Oryza sativa). (Dr. Maiale).

Defense mechanisms in rapeseed against the phytopathogenic fungus Phoma lingam (Dr. Rossi).

Microbial endophytes as a biotechnological alternative for the control of disease in horticultural brassica species. The role of the microbial polyamine metabolism in the colonization of the plant apoplast and the control of diseases (Dr. Romero)

Publications

2024 Publications

2023 Publications

2022 Publications

Campestre MP, Antonelli CJ, Bailleres MA, Gortari M, Maguire VE, Taboada MA, Ruiz OA. Lotus tenuis biological nitrogen fixation and performance contribute to defining its strategic role in the Salado River Basin lowlands (Argentina). Agriculture, Ecosystems & Environment 340, 108159. 2022. https://doi.org/10.21203/rs.3.rs-2037673/v1

Dinolfo MI, Martínez M, Castañares E, Vanzetti LS, Rossi F, Stenglein SA, Arata AF. Interaction of methyl-jasmonate and Fusarium poae in bread wheat. Fungal Biology 126, 786-798. 2022. https://doi.org/10.1016/j.funbio.2022.10.002

Duhalde MA, Bezus R, Rodríguez AA, Maiale SJ, Romero FM. Optimization of tissue culture conditions and in vitro and in planta transformation of the local variety of rice Don Justo FCAyF. Revsita Facultad Agronomía, Biotecnologías Aplicadas a Cultivos de Interés Socioeconómico 121, 1-13. 2022. https://doi.org/10.24215/16699513e100

Liebrenz K, Gómez C, Brambilla S, Frare R, Stritzler M, Maguire V, Ruiz O, Soldini D, Pascuan D, Soto G, Ayub N. Whole-Genome resequencing of spontaneous oxidative stress-resistant mutants reveals an antioxidant system of Bradyrhizobium japonicum involved in soybean colonization. Microbial Ecology 84, 113-1140.2022. https://doi.org/10.1007/s00248-021-01925-2

Maguire VG, Rodríguez AA, Ezquiaga JP, Salas N, Gortari M, Ayub N, Bouilly PJ. Romero MF, Gárriz A, Ruiz OA. Analysis of the contribution of Lotus corniculatus to soil carbon content in a rice-pasture rotation system. Agriculture, Ecosystems and Environment 340, 108159. 2022. https://doi.org/10.1016/j.agee.2022.108159

Salas N, Cóceres MV, dos Santos Melo T, Pereira-Neves A, Maguire VG, Rodriguez T, Sabatke B, Ramirez MI, Sha J, Wohlschlegel JA, de Miguel N. VPS32, a member of the ESCRT complex, modulates adherence to host cells in the parasite Trichomonas vaginalis by affecting biogenesis and cargo sorting of released extracellular vesicles. Cellular and Molecular Life Sciences 79, 11. 2022. https://doi.org/10.1007/s00018-021-04083-3

Solmi L, Rosli HG, Pombo MA, Stadler S, Rossi FR, Romero FM, Ruiz OA, Gárriz A. Inferring the role of the metabolism of polyamines in the phytopathogenic bacteria Pseudomonas Syringae: a meta-analysis approach Frontiers in Microbiology 13, 893626. 2022. https://doi.org/10.3389/fmicb.2022.893626

Zhang X, Pramod K, Puchalski P, Leehan JD, Rossi FR, Okumoto S, PIlot G, Danna CH. MAMP-elicited changes in amino acid transport activity contribute to restricting bacterial growth. Plant Physiology 189, 2315-2331. 2022. https://doi.org/10.1093/plphys/kiac217

2021 Publications

Antonelli CJ, Calzadilla PI, Campestre MP, Escaray FJ, Ruiz OA. Contrasting response of two Lotus corniculatus L. accessions to combined waterlogging–saline stress. Plant Biology 23, 363–374. 2021. https://doi.org/10.1111/plb.13216

Cumpa-Velásquez LM, Moriconi JI, Dip DP, Castagno LN, Puig ML, Maiale SJ, Santa María GE, Sannazzaro AI, Estrella MJ. Prospecting phosphate solubilizing bacteria in alkaline-sodic environments reveals intra-specific variability in Pantoea eucalypti affecting nutrient acquisition and rhizobial nodulation in Lotus tenuis. Applied Soil Ecology 168, 104125. 2021. https://doi.org/10.1016/j.apsoil.2021.104125

Mantz GM, Rossi FR, Viretto PE, Noelting MC, Maiale SJ.Stem canker caused by Phomopsis spp. Induces changes in polyamine levels and chlorophyll fluorescence parameters in pecan leaves. Plant Physiology and Biochemistry 166, 761-769. 2021. https://doi.org/10.1016/j.plaphy.2021.06.050

Menéndez AB, Ruiz OA. Stress-regulated elements in Lotus spp., as a possible starting point to understand signalling networks and stress adaptation in legumes. PeerJ – Life and Environment. 9, e12110. 2021. https://doi.10.7717/peerj.12110

Nieva AS, Romero FM, Erban A, Carrasco P, Ruiz OA, Kopka J. Metabolic profiling and metabolite correlation network analysis reveal that Fusarium solani Induces differential metabolic responses in Lotus japonicus and Lotus tenuis against severe phosphate starvation. Journal of Fungi 7, 765. 2021. https://doi.org/10.3390/jof7090765

Puig ML, Rodríguez AA, Vidal AA, Bezus R, Maiale SJ. Patterns of physiological parameters and nitrogen partitioning in flag leaf explain differential grain protein content in rice. Plant Physiology and Biochemistry 168, 457-464. 2021. https://doi.org/10.1016/j.plaphy.2021.10.034

Rossi FR, Gárriz A, Marina M, Pieckenstain FL. Modulation of polyamine metabolism in Arabidopsis thaliana by salicylic acid. Physiologia Plantarum 173, 843-855. 2021. https://doi.org/10.1111/ppl.13478

2020 Publications

2019 Publications

Acevedo RM, Avico EH, González S, Salvador AR, Rivarola M, Paniego N, Nunes-Nesi A, Ruiz OA, Sansberro PA. Transcript and metabolic adjustments triggered by drought in Ilex paraguariensis leaves. Planta. 250: 445-462. 2019. https://doi.org/10.1007/s00425-019-03178-3

Antonelli CJ, Calzadilla PI, Vilas JM, Campestre MP, Escaray FJ, Ruiz OA. Physiological and anatomical traits associated with tolerance to long-term partial submergence stress in the Lotus genus: responses of forage species, a model and an interspecific hybrid. J Agron Crop Sci. 205: 65-76. 2019. https://doi.org/10.1111/jac.12303

Bordenave CD, Granados Mendoza C, Jiménez Bremont JF, Gárriz A, Rodríguez AA. Defining novel plant polyamine oxidase subfamilies through molecular modeling and sequence analysis. BMC Evol Biol. 19: 28. 2019. https://doi.org/10.1186/s12862-019-1361-z

Bordenave CD, Rocco R, Maiale SJ, Campestre MP, Ruiz OA, Rodriguez AA, Menendez AB. Chlorophyll a fluorescence analysis reveals divergent photosystem II responses to saline, alkaline and saline–alkaline stresses in the two Lotus japonicus model ecotypes MG20 and Gifu-129. Acta Physiol Plant. 41: 167. 2019. https://doi.org/10.1007/s11738-019-2956-0

Calzadilla PI, Vilas JM, Escaray FJ, Unrein F, Carrasco P, Ruiz OA. The increase of photosynthetic carbon assimilation as a mechanism of adaptation to low temperature in Lotus japonicus. Sci Rep. 9: 863. 2019. https://doi.org/10.1038/s41598-018-37165-7

Escaray FJ, Antonelli CJ, Carrasco P, Ruiz OA. Interspecific hybridization improves the performance of Lotus spp. under saline stress. Plant Sci. 283: 202-210. 2019. https://doi.org/10.1016/j.plantsci.2019.02.016

Escaray FJ, Antonelli CJ, Copello GJ, Puig S, Peñarrubia L, Ruiz OA, Perea-García A. Characterization of the copper transporters from Lotus spp. and their Involvement under flooding conditions. Int J Mol Sci. 20: 3136. 2019. https://doi.org/10.3390/ijms20133136

Espasandin FD, Brugnoli EA, Ayala PG, Ruiz OA, Sansberri P. Long-term preservation of Lotus tenuis adventitious buds. Plant Cell Tiss Organ Cult. 136: 373-382. 2019. https://doi.org/10.1007/s11240-018-1522-6

Gázquez A, AbdElgawad H, Baggerman G, Van Raemdonck G, Asard H, Maiale SJ, Rodríguez AA, Beemster GTS. Redox homeostasis in the rice leaf growth zone plays a key role in cold tolerance. J Exp Bot erz455. 2019. https://doi.org/10.1093/jxb/erz455

Menéndez AB, Calzadilla PI, Sansberro PA, Espasandin FD, Gazquez A, Bordenave CD, Maiale SJ, Rodríguez AA, Maguire VG, Campestre MP, Garriz A, Rossi FR, Romero FM, Solmi L, Salloum MS, Monteoliva MI, Debat JH, Ruiz OA. Polyamines and Legumes: Joint Stories of Stress, Nitrogen Fixation and Environment. Front Plant Sci 10: 1415. 2019. https://doi.org/10.3389/fpls.2019.01415

Nieva AS, Vilas JM, Gárriz A, Maiale SJ, Menéndez AB, Erban A, Kopka J, Ruiz OA. The fungal endophyte Fusarium solani provokes differential effects on the fitness of two Lotus species. Plant Physiol Biochem. 144: 100-109. 2019. https://doi.org/10.1016/j.plaphy.2019.09.022

Pires N, Maiale S, Venturino A, Lascano C. Differential effects of azinphos-methyl and chlorpyrifos on polyamine oxidative metabolism during the embryonic development of Rhinella arenarum and its relation to oxidative stress. Pestic Biochem Phys. En Prensa. 2019. https://doi.org/10.1016/j.pestbp.2019.10.007

Romero FM, Gatica-Arias A. CRISPR/Cas9: Development and Application in Rice Breeding. Rice Sci. 26: 265-281. 2019. https://doi.org/10.1016/j.rsci.2019.08.001

Romero FM, Rossi FR, Gárriz A, Carrasco P, Ruíz OA. A bacterial endophyte from apoplast fluids protects canola plants from different phytopathogens via antibiosis and induction of host resistance. Phytopathology 109: 375-383. 2019. https://doi.org/10.1094/PHYTO-07-18-0262-R